An Assessment of the Clinical Value of a Canine Blood Typing Kit (DEA1.1) Based on Erythrocyte Agglutination

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  • 赤血球凝集反応を利用した犬用血液型（DEA1.1型）判定試薬の臨床的有用性の評価

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The clinical value of a canine blood type grouping kit was evaluated. This type of kit is designed to check erythrocyte agglutination with DEA (Dog Erythrocyte antibody) 1.1 monoclonal antibodies. In 79 hematologically normal dogs, 57 were determined to be DEA1.1 positive (72.2%), and the remaining 22 dogs were determined to be DEA1.1 negative. Eighteen of the DEA1.1 positive dogs and 6 negative dogs were selected at random, and the same sample from each animal was tested five times, and showed excellent reproducibility. Ten DEA1.1 positive and 10 negative blood samples from randomly selected dogs were matched by a conventional method, cross matching with one sample of DEA1.1 positive blood. There was no agglutination seen in any case, which confirmed that it is impossible to distinguish DEA1.1 positive and negative by crossmatching. From these findings, it is concluded that this kind of canine blood typing kit is useful in judging DEA1.1 types easity and rapidly. In clinics, the kit would be of much use as a compatibility test before transfusinon.

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