卵黄生成に関する生化学的研究

DOI

書誌事項

タイトル別名
  • Biochemical Studies on Egg Yolk Formation in the Domestic Fowl
  • II. Fractionation of Serum Phosphoproteins
  • II. 血清燐蛋白の分離

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抄録

The blood sera of the laying hen, estrogen-treated and non-treated cockerels were dialyzed against 0.015M sodium phosphate buffer, pH6.8, at 2°C for 24 hours. The resulting preci-pitated globulin was separated by centrifugation, re-dialyzed, and lyophilized. Four milliliters of supernatant serum and about 0.1g of lyophilized globulin were applied separately to a column which had been filled with a suspension of DEAE-cellulose in 0.015M sodium phosphate buffer, pH6.8, and the content of which had been compressed with air to a constant height 1×20cm.<br>Stepwise elution was carried out by 0.013M sodium phosphate buffer, pH6.8, containing various concentrations of sodium chloride. Each protein fraction was read for protein concentration with a spectrophotometer in the ultraviolet at 280mμ.<br>The chromatography of globulin of the laying hen and that of the estrogen-treated cockerel showed four fractions, which were not observed in globulin of the non-treated cockerel. These four fractions were eluted by 0.3, 0.4, 0.5, and 2.0M sodium chloride containing buffer and found to have 6.7, 2.4, 2.7, and 16.0% protein-bound phosphorus in the laying hen and 6.1, 3.6, 7.0, and 17.1% in the estrogen-treated cockerel, respectively. The ratio of the total amount of the four fractions to the precipitated globulin was 6.3% in the laying hen and 2.7% in the estrogen-treated cockerel. These four fractions seemed to be phosphoprotein.

収録刊行物

  • 日本畜産学会報

    日本畜産学会報 33 (3), 249-255, 1962

    公益社団法人 日本畜産学会

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