卵黄生成に関する生化学的研究
書誌事項
- タイトル別名
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- Biochemical Studies on Egg Yolk Formation in the Domestic Fowl
- II. Fractionation of Serum Phosphoproteins
- II. 血清燐蛋白の分離
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The blood sera of the laying hen, estrogen-treated and non-treated cockerels were dialyzed against 0.015M sodium phosphate buffer, pH6.8, at 2°C for 24 hours. The resulting preci-pitated globulin was separated by centrifugation, re-dialyzed, and lyophilized. Four milliliters of supernatant serum and about 0.1g of lyophilized globulin were applied separately to a column which had been filled with a suspension of DEAE-cellulose in 0.015M sodium phosphate buffer, pH6.8, and the content of which had been compressed with air to a constant height 1×20cm.<br>Stepwise elution was carried out by 0.013M sodium phosphate buffer, pH6.8, containing various concentrations of sodium chloride. Each protein fraction was read for protein concentration with a spectrophotometer in the ultraviolet at 280mμ.<br>The chromatography of globulin of the laying hen and that of the estrogen-treated cockerel showed four fractions, which were not observed in globulin of the non-treated cockerel. These four fractions were eluted by 0.3, 0.4, 0.5, and 2.0M sodium chloride containing buffer and found to have 6.7, 2.4, 2.7, and 16.0% protein-bound phosphorus in the laying hen and 6.1, 3.6, 7.0, and 17.1% in the estrogen-treated cockerel, respectively. The ratio of the total amount of the four fractions to the precipitated globulin was 6.3% in the laying hen and 2.7% in the estrogen-treated cockerel. These four fractions seemed to be phosphoprotein.
収録刊行物
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- 日本畜産学会報
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日本畜産学会報 33 (3), 249-255, 1962
公益社団法人 日本畜産学会
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詳細情報 詳細情報について
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- CRID
- 1390001205191249664
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- NII論文ID
- 130000740582
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- ISSN
- 18808255
- 1346907X
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
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- 抄録ライセンスフラグ
- 使用不可