Free amino acids in blastocoelic fluid, uterine fluid and blood serum of the rabbit

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  • SUGAWARA Shichiro
    Laboratory of Animal Reproduction, Faculty of Agriculture, Tohoku University

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  • ウサギ胚盤胞腔液の遊離アミノ酸組成
  • ウサギハイバンホウコウエキ ノ ユウリ アミノサン ソセイ

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Free amino acids of blastocoelic fluid, uterine fluid, maternal serum and peritoneal fluid before and during implanation or following ovariectomy were estimated by amino acid autoanalyser (KLA-3B type Hitachi Ltd.).<br>The total of 18 free amino acids were identified both in blastocoelic fluid of blastocysts from 6 to 8 days p. c. (post coitus) and in the uterine fluids.<br>The major amino acids identified in blastocoelic fluid were glycine, alanine, serine, threonine, glutamic acid, valine and they occupied over 90% of total free amino acid estimated.<br>The concentrations of the total and of most of the individual free amino acids in blastocoelic fluid were greater than those in uterine fluid, peritoneal fluid and maternal serum. In 6 and 7 day blastocyst, the concentration of glycine and alanine was nine and sixteen times higher than the concentration found in the maternal serum and peritoneal fluid.<br>In the blastocoelic fluid from the blastocysts before implantation stage (6, 7 days p. c.), the concentration of most of free amino acids was, in general, greater than the concentration estimated in the blastocyst during implantation from 7 1/2 to 8 days p. c.<br>During implantation stage, the concentration of these amino acids in blastocoelic fluid decreased and was comparable to their level in maternal fluid.<br>Ovariectomy at 6 days p. c. had an effect on the concentration of glycine, serine, glutamic acid, arginine, aspartic acid and lysine in the blastocoelic fluid at 7 days p. c. While the concentration of alanine, threonine and valine did not alter following ovariectomy. The biological significance of free amino acid in blastocyst was discussed in comparison with the uterine fluid, maternal serum and ovarian function.

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