ハムスター卵子におけるΔ<sup>5</sup>-3β-ハイドロオキシステロイド脱水素酵素の組織化学的研究

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タイトル別名
  • Histochemical Studies of Δ<sup>5</sup>-3β-Hydroxysteroid Dehydrogenase in Hamster Eggs
  • ハムスター ランシ ニ オケル デルタ5 3ベータ ハイドロオキシステロイド

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The activity of Δ5-3β-hydroxysteroid dehydrogenase in hamster eggs at the preimplantation period was investigated by a histochemical method using 17α-hydroxypregnenolone as a substrate. Eggs were obtained at different developmental stages: unfertilized eggs 2 hours after ovulation, sperm-penetrated eggs 4 hours, pronuclear eggs 11 hours, 2-cell eggs 32 hours, 4-cell eggs 52 hours, 8-cell eggs 65 hours, and blastocysts 76 hours. Histochemical demonstration of the enzyme was made by the Dickmann and Dey method for steroid dehydrogenase. The composition of the medium was 1.8mg 17α-hydroxypregnenalone, 4.0mg NAD, 2.0mg Nitro-BT and 10ml 0.1M phosphate buffer solution. A strong enzyme activity was observed in unfertilized, sperm-penetrated and pronuclear eggs; diformazan granules were distributed evenly throughout the cytoplasm of the unfertilized and sperm-penetrated eggs;while in the pronuclear eggs, they were especially abundant in the perinuclear region. The intensity of the enzyma activity was retained until the eggs reached the 4-cell stage, but was then weakened at the 8-cell stage. In cleaved eggs, diformazan granules were especially abundant in the perinuclear region and the outer layer of the cytoplasm. No enzyme activity was found in the blastocysts. From these results, it seems that conversion of 17α-hydroxypregnenolone to 17α-hydroxyprogesterone occurs in hamster eggs at the 1-to 8-cell stages, but does not occur in the blastocysts.

収録刊行物

  • 日本畜産学会報

    日本畜産学会報 48 (3), 173-176, 1977

    公益社団法人 日本畜産学会

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