ハムスターにおける卵胞卵子のステロイド代謝に関する組織化学的研究

書誌事項

タイトル別名
  • Histochemical Studies on Steroid Synthesis in Follicular Eggs of the Hamster
  • ハムスター ニ オケル ランポウ ランシ ノ ステロイド タイシャ ニ カンス

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説明

Histocheical demonstration of Δ5-3β-hydroxysteroid dehydrogenase (Δ5-3β-HSD), NADH2 dehydrogenase (NADH2-DH), NADPH2 dehydrogenase (NADPH2-DH) and glucose-6-phosphate dehydrogenase (G-6-PDH), as well as electron-histochemical demonstration of adenylate cyclase, was performed on hanster eggs in primordial through Graafian foliicles. The activity of Δ5-3β-HSD first came to appear in the eggs in large secondary follicles, getting stronger in those in Graafian follicles. The activities of NADH2-DH, NADPH2-DH and G-6-PDH first appeared in the eggs in small secondary follicles: the activities of the former two enzymes became gradually stronger along with follicular growth, while that of the last was always strong. A moderate activity of adenylate cyclase was recognised as lead granules on the cytoplasmic membrane of eggs in small secondary through Graafian follicles. These results suggest that (1) steroid synthesis starts in hamster eggs when they are in large secondary follicles, and (2) NADPH2 produced by G-6-PDH is used for steroid synthesis in the eggs.

収録刊行物

  • 日本畜産学会報

    日本畜産学会報 51 (3), 192-196, 1980

    公益社団法人 日本畜産学会

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