山羊精子の嫌気的解糖および生存性に及ぼす炭酸ガスの影響

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タイトル別名
  • Effect of Carbon Dioxide on Anaerobic Glycolysis and Motility of Goat Spermatozoa
  • ヤギ セイシ ノ ケンキテキ カイトウ オヨビ セイゾンセイ ニ オヨボス タ

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抄録

Experiments were carried out to determine the effect of CO2 on the anaerobic glycolysis and motility of goat spermatozoa. Twice washed spermatozoa were incubated at 37°C under anaerobic conditions containing various levels of CO2. Calcium-free Krebs Ringer bicarbonate solution was used as a basic medium for incubation. 1) Anaerobic glycolysis of spermatozoa was depressed with the increase in pCO2 up to the maximum inhibition (60-70%)which occurred under 100%CO2. However, incubation of spermatozoa under high pCO2 was not harmful to their motility. 2) Inhibition of sperm metabolism by CO2 was reversible, and spermatozoa exhibited normal glycolytic and respiratory activity during subsequent incubation under low pCO2 after transfer from the 2-hr incubation under high pCO2. 3) In the media containing enough bicarbonate or trishydroxymethylaminomethane for the maintenance of pH at approximately 7.0 under saturation with CO2, inhibition of glycolysis by CO2 was almost similar to that observed in a control medium in which the pH dropped to 6.2-6.3; this suggests that the inhibitory effect of CO2 on glycolysis is not directly due to the drop of extracellular pH.

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  • 日本畜産学会報

    日本畜産学会報 48 (12), 715-720, 1977

    公益社団法人 日本畜産学会

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