反芻胃内繊毛虫の細胞外および細胞内蛋白質分解活性の性質

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タイトル別名
  • On some Properties of Extra and Intracellular Proteolytic Activity of Rumen Ciliate Protozoa
  • ハンスウイナイ センモウチュウ ノ サイボウガイ オヨビ サイボウナイ タンパ

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Experiments were done to clarify some properties of extra and intracellular proteolytic enzymes of rumen ciliate protozoa, The rumen ciliate protozoa were collected from the rumen contents taken through fistula attached to the rumen of goats and the crude enzyme solutions were prepared from the collected ciliates. The crude extracellular enzyme solutions were fractionated by salting out with ammonium sulfate and its 40-80% saturated fraction was furthermore fractionated by using Sephadex G-100 column. The properties of these crude proteolytic enzymes are summarized as follows: 1. The optimum temperature of the crude extracellular enzymes was 55-60°C and the optimum pH was 5.5-6.0. 2. The optimum temperature of the crude intracelIular enzymes was 70-75°C and the optimum pH was 5.0-6.0. 3. The proteolytic activity of the crude extracellular enzymes per protein nitrogen in the solution was much stronger than that of the crude intracellular enzymes. 4. About 80% of total proteolytic activity of the crude extracellular enzymes was detected in the fraction precipitated by 40-80% ammonium sulfate saturation. 5. The proteolytic activity of the extracellular enzymes was markedly increased by cysteine or dithiothreitol. 6. Gel filtration of the ammonium sulfate fraction (40-80% saturation) on Sephadex G-100 revealed 2-4 main peaks of proteolytic activity.

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  • 日本畜産学会報

    日本畜産学会報 52 (12), 861-868, 1981

    公益社団法人 日本畜産学会

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