細胞外蛋白質分解酵素を産生する反芻胃内繊毛虫の種類とその活性に及ぼす細菌の影響

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  • Species of Rumen Ciliate Protozoa Which Produce Extra-Cellular Protease and Influence of Bacteria on Its Activity
  • サイボウガイ タンパクシツ ブンカイ コウソ オ サンセイスル ハンスウ イナ

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In vitro experiments were done in order to identify the species of rumen ciliate protozoa which produce proteolytic enzymes outside the cell and the effect of contaminated bacteria on the extra-cellular proteolytic activity. Bacteriaand ciliate protozoa were isolated from the rumen liquid obtained from a Holstein cow through the fistula, and protozoa were separated into two groups ofentodiniomorphs and holotrichs. The extra-cellular crude enzyme solutions wereprepared by centrifuging the starved culture medium of each protozoa group andof isolated bacteria, and the proteolytic activity was assayed through thedecomposition of casein incubated with crude enzyme preparations. The resultsobtained are summarized as follows: Extra-cellular proteolytic activity wasdetected exclusively in the culture medium of entodiniomorphs, and not in themedium of holotrich protozoa. Isolated bacteria decomposed casein, but theproteolytic.activity was hardly detected in the cell-free medium prepared after theculture of bacterial suspension. Extra-cellular proteolytic activity detected forentodiniomorphid protozoa was not modified by washing these organisms severaltimes with surface-active substance (sugar-fatty acid ester) to eliminate bacteria, which may attach to the surface of ciliate protozoa. the results of this worksuggest that the extra-cellular proteolytic activity would be principally attributedto entodiniomorphid protozoa, and that the influence of bacteria could beneglected.

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