lmmunocytochemical and Ultrastructural Studies of Prolactin (PRL) Cells in Gifu Native Fowls

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  • 岐阜地鶏Prolactin(PRL)細胞の免疫細胞化学的および微細構造学的研究
  • ギフ ジドリ Prolactin PRL サイボウ ノ メンエキ サイボウ カ

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Abstract

The purpose of this study was to identify PRL cells in Gifu native fowls which retain intensive broodiness by an immunocytochemical technique and to demonstrate their ultrastructural characteristics. By light microscopic immunocytochemistry using an peroxidase-antiperoxidase method, it was confirmed that PRL cells were located at the cephalic lobe and increased markedly in number in broody hens. By electron microscopic immunocytochemistry using an immunoglobulin-gold complex method, it was demonstrated that PRL cells contained round or oval secretory granules of low density with diameters ranging from 200-300nm in laying hens and 250-500 or 600nm in broody hens. The ultrastructures of PRL cells in the broody hens were characterized by their well developed cell organelles, i. e. densely packed rough endoplasmic reticulum with parallel arranged lamellae, abundant free or attached ribosomes and prominent Golgi apparatus actively forming the granules. These findings suggest high protein synthesis and active hormone secretion of PRL cells during the broody period.

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