精巣上体内成熟過程における豚精子抗原性の変化

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書誌事項

タイトル別名
  • Antigenic Changes in the Protein of Boar Spermatozoa during Maturation in Epididymis
  • セイソウ ジョウタイ セイジュク カテイ ニ オケル ブタ セイシ コウゲンセ
  • 1. Double Immunodiffusion Analyses
  • 1.二重拡散法による分析

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抄録

Testes were removed immediately post mortem from 20 almost sexually matured Landrace boars. Spermatozoa and fluids were collected with a cannula from the caput, corpus and cauda epididymidis and vas deferens. Semen samples from a mature Landrace boar aged 2.0 years were also subjected to study. Using five rabbit anti-boar sperm sera, the antigenicity of spermatozoa and fluids from the epididymis, vas deferens and ejaculates were examined by double mmunodiffusion analyses including an absorption test. Three specific proteins represented by precipitin lines were detected in sperm antigen. Of these, one antigenic component was common to sperm from epididymis, vas deferens and ejaculates. It is suggested that this antigen was sperm coating antigen of testicular or caput epididymal origin. Another antigenic component revealed in the caput was not detected in spermatozoa from the cauda epididymidis, vas deferens or ejaculates. Such antigenic changes of spermatozoa seemed to be functionally related to the maturation of boar spermatozoa in the corpus epididymidis. Six specific proteins represented by precipitin lines were found in fluids and seminal antigens with the five sperm antisera. Two antigenic components could not be detected in epididymal and vas deferens fluids, being present only in seminal plasma against sperm antisera. No precipitin lines occurred as a result of spur reaction for epididymal, vas deferens and ejaculated sperm antigens, the majority of the lines being due to coalescence or partial identity. Conversly, no coalescence lines were detected for fluids and seminal plasma antigens, which showed spur reaction or partial identity, thus confirming that ejaculated spermatozoa are coated in part with antigens probably contributed by the vas deferens or male accessory glands.

収録刊行物

  • 日本畜産学会報

    日本畜産学会報 57 (2), 131-138, 1986

    公益社団法人 日本畜産学会

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