Effect of Peptides and Amino Acids Produced by Lactobacillus casei in Milk on the Acid Production of Bifidobacteria

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  • CFENG Cheng-Chuan
    Department of Agricultural Chemistry, Faculty of Agriculture, Tamagawa University
  • NAGASAWA Taro
    Department of Agricultural Chemistry, Faculty of Agriculture, Tamagawa University

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  • Lactobacillus caseiの生産するペプチドおよびアミノ酸がビフィズス菌の生酸性に及ぼす影響
  • Lactobacillus casei ノ セイサンスル ペプチド オヨビ ア

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Abstract

It was found that the growth of bifidobacteria was greatly stimulated in the mixed incubation with Lactobacillus casei. Hence the peptides and free amino acids isolated from the culture filtrate prepared from the incubated milk with L. casei were investigated in relation to the stimulation on the acid production of bifidobacteria. Bifidobacterium breve, B. infantis, and Lactobacillus casei were used as test organisms. The preparation of culture filtrate was performed by means of DAHIYA and SPECK, and the removal of free amino acids from culture filtrate by the method of MARKOVITZ and STEINBERG. The main results obtained were as follows: 1. Additions of the culture filtrate containing peptides and amino acids prepared from the incubated milk with L. casei and the free amino acids-removed culture filtrate gave appreciable stimulation for the acid production of bifidobacteria over those of control, and the degree of stimulation was maximum with the addition of untreated filtrate at 24hr of incubation. But little difference was observed in the stimulation between two filtrates after 48hr of incubation. 2. In the case of the addition of single amino acid or the omission in turn of single amino acid from a mixture of amino acids, the stimulation on the acid production of bifidobacteria was observed to be highest for cystine among 18 amino acids, followed by methionine, lysine, alanine, and histidine. 3. The stimulatory effect by cystine was greater in B. infantis than in B. breve. Isoleucine or tryptophan gave not only the reduction of acid production of B. infantis but also considerably reduced the stimulation by cystine with the increasing amount of isoleucine or tryptophan. 4. Active fractions, having stimulation on the acid production, obtained from gel filtration of the free amino acids-removed culture filtrate with Sephadex G-15 contained five peptides, ranging from 948 to 2, 319 in molecular weights. Lys, Met, Ala, Leu and Val of the amino acids detected in the peptides were considered to stimulate the acid production of bifidobacteria.

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