On Normality of Equine Transferrin Contents in General Population and Quantitative Difference of Transferrin Protein of Normal Homozygote and Heterozygote with Silent Gene

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  • 馬のTf蛋白量の一般集団における正常値の分布並びにTfのホモ型とsilent geneの関与するヘテロ型の蛋白量の差異について
  • ウマ ノ Tf タンパクリョウ ノ イッパン シュウダン ニ オケル セイジョ

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The intensities of transferrin bands having silent gene (abnormal transferrin type) reported in our previous paper were a little weaker than those of normal bands of homozygotes. From this phenomenon, it was presumed that a quantitative difference of transferrin protein might exist between normal and abnomal transferrin types. This difference, however, could not be definitely distinguished by starch gel electrophoresis. This work was carried out to establish a single radial immunodiffusion method by the use of our anti-Tf serum which was produced to measure the amount of horse serum transferrin protein, and thus to research the distribution of the amount of transferrin protein in normal horse population. At the same time, it was attempted to distinguish abnormal transferrin types from normal ones by measuring their transferrin proteins. The results are as the following: 1. When a single radial immunodiffusion test was carried out by anti-Tf serum which had titer from 1:32 to 1:64, following was the best conditions for the test: 1), 0.25ml of anti-Tf serum is mixed with 10ml of agar solution, 2), 3μl of antigen solution is poured into well of 3.0mm in diameter and 3), the agar plate of 16×11.5cm (using 20ml of antiserum-agar solution) is then placed horizontally in chamber with high humidity at 37°C for 72 hours. 2. It was recognized that the liner relationship existed between the square of diameter of final precipitate ring and dilution of the reference transferrin protein (42.5-340mg/dl). The coefficient of variation of the measurements to an amount of antigen was less than 1. 19 per cent. Therefore, it was easy to measure the amount of transferrin protein in the horse serum with this standard curve. 3. The amount of transferrin protein in normal population of 621 light breed horses of over 4 years old was observed to distribute from 241. 4 to 506. 6mg/dl, and the mean value was 352.8mg/dl. Also, the normality of transferrin contents in horse serum was recognized at 1% level. 4. The amount of transferrin protein in abnormal transferrin types showed low transferrin level, and these were not included in the distribution of normal value of transferrin contents. The mean value of the transferrin content in 5 abnormal transferrin types was 198. 6mg/dl, which was about 150mg/dl less than that of normal transferrin types. Therefore, it became possible technically to distinguish abnormal transferrin types from normal ones with the single radial immunodiffusion method.

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