DNA Fingerprint Analysis of Cattle using Bacteriophage M 13 Repetitive Sequence as a Probe

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  • MANNEN Hideyuki
    The Graduate School of Science and Technology and, Kobe University
  • TSUJI Soichi
    Laboratory of Animal Breeding, Faculty of Agriculture, Kobe University
  • MUKAI Fumio
    Laboratory of Animal Breeding, Faculty of Agriculture, Kobe University
  • GOTO Nobuo
    Laboratory of Animal Breeding, Faculty of Agriculture, Kobe University

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Other Title
  • M13ファージ反復配列をプローブとしたウシのDNAフィンガープリント分析
  • M13 ファージ ハンプク ハイレツ オ プローヴ ト シタ ウシ ノ DNA

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Abstract

A DNA fingerprint analysis of cattle using the repetitive sequence of M 13 phage DNA as a probe revealed 15 to 22 bands, ranging between 1 and 20 kilobases in size, in each individual examined. DNA fingerprints of three cattle breeds, Japanese Black, Japanese Brown and Holstein, showed high variability and specific banding pattern for each individual. The probabilities of shared band (Sb) for DNA fingerprints to estimate genetic similarity between individuals were 0.659±0.093 for Japanese Black, 0.714±0.056 for Japanese Brown, 0.643±0.155 for Holstein, and the Sb between breeds were 0.580±0.068 for Japanese Black and Japanese Brown, 0.520±0.056 for Japanese Black and Holstein, and 0.535±0.079 for Japanese Brown and Holstein. DNA fingerprint analysis of four Japanese Black families showed that all bands of offspring originated from those of either parent. The number of common bands between individuals increased with increasing coefficient of relationship between them. These results indicate that DNA fingerprinting using M 13 phage repetitive sequence is a powerful tool for identifying individuals and parentage test in cattle.

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