Studies on Cytotoxic Properties of Japanese Quail Ovotransferrin
-
- ODASHIMA Mari
- The United Graduate School of Agricultural Science, Gifu University (Shinshu University)
-
- OTANI Hajime
- Faculty of Agriculture, Shinshu University
Bibliographic Information
- Other Title
-
- ウズラオボトランスフェリンの細胞傷害特性
Abstract
Cytotoxic properties of Japanese quail ovotransferrin were estimated by trypan blue exclusion. The ovotransferrin revealed a cytotoxicity toward C3H/HeN mouse T-lymphocytes, mouse B-lymphocytes and Jurkat Clone E6-1 cells (a human cell line derived from acute leukemia T-lymphocyte), while it was inert to C3H/HeN mouse plastic-adherent cells and Japanese quail spleen cells. DNA fragmentation was observed on terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling and agarose gel electrophoresis when mouse spleen lymphocytes were cultured with quail ovotransferrin for 12h. On the other hand, the cytotoxic activity of ovotransferrin diminished completely when the protein was treated with PNGase F or pepsin, whereas a strong cytotoxic activity remained after tryptic digestion of iron-saturated quail ovotransferrin. A cytotoxic glycoprotein having molecular weights of 39, 000 was obtained from the trypsin digest. These results indicate that Japanese quail ovotransferrin may induce apoptosis to mouse spleen T-and B-lymphocytes and Jurkat cells, but not to quail spleen lymphocytes and mouse monocytes/macrophages. Moreover, these results suggest that the cytotoxic activity is attributable to C-lobe in quail ovotransferrin.
Journal
-
- Nihon Chikusan Gakkaiho
-
Nihon Chikusan Gakkaiho 71 (9), 338-346, 2000
Japanese Society of Animal Science
- Tweet
Details 詳細情報について
-
- CRID
- 1390001205194103296
-
- NII Article ID
- 130000742460
-
- ISSN
- 18808255
- 1346907X
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed