Staphylococcus aureus感染牛における乳房炎検査成績の変動

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  • Variation in the Results of Bacteriologic Culturing and Indirect Diagnostic Methods for <i>Staphylococcus aureus</i> Infected Udder over a Two Month Period
  • Staphylococcus aureus カンセン ウシ ニ オケル ニュウ

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Mastitis infection is usually confirmed by bacteriologic culturing, but several indirect diagnostic methods are widely used as indicators of mastitis to simplify the procedure and lower the cost. These methods include the measuring of somatic cell counts(SCC), electrical conductivity (EC) and differential value (DEC), N-acetyl-β-D-glucosaminidase (NAGase) activity. However, the diagnosis sometimes differs depending on the indicator used and the time of sampling. To clarify weekly changes in the results of indicators in a mastitis quarter, ten S. aureus infected quarters from 15 milking cows and quarter milk samples from them were examined weekly in order to determine SCC, EC, DEC and NAGase over an eight week period. The ten quarters had been confirmed as S. aureus infected by bacteriologic culturing, and detection of the anti-S. aureus antibody in milk had been confirmed by enzyme-linked immunosorbent assay (ELISA). Though the results of each examination showed considerable fluctuation according to the week, parallel movement patterns were generally observed. However, neither positive results of culture nor ELISA correlation with the patterns were found. When the guideline for mastitis treatment proposed by a government committee was applied to the results of 80 examinations (10 quarters×8 times), percentages exceeding the criteria were 92.5% for SCC, 85.0% for NAGase, 71.2% for EC and 56.2% for DEC. ELISA was useful as a screening test for detecting an S. aureus infected quarter, being more effective for detection than culturing. The determination of SCC and NAGase, not only once but several times, would be the most reliable method for confirming mastitis infection since they are effective and convenient.

収録刊行物

  • 日本畜産学会報

    日本畜産学会報 64 (5), 462-469, 1993

    公益社団法人 日本畜産学会

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