<i>In vitro</i>におけるルーメンプロトゾアによるルーメン真菌のキチンおよびタンパク質代謝

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タイトル別名
  • <i>In vitro</i> Metabolism of Chitin and Protein from Ruminal Fungi by Ruminal Protozoa
  • In vitro ニ オケル ルーメン プロトゾア ニ ヨル ルーメン シンキ

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The predation of ruminal anaerobic fungi by ruminal protozoa was studied using an in vitro system. Two fungi Neocallimastix sp. and Piromyces sp., were isolated from a goat. Mixed rumen ciliate protozoa obtained from goats and suspended in MB 9 buffer solution were incubated anaerobically with each freeze-dried fungus at 39°C for up to 24h. The total amount of chitin in cultures, and glucosamine and amino acids released in the medium were monitored. The total amount of chitin through the incubation period tended to decrease in the case of Piromyces sp. and in the case of Neocallimastix sp. it decreased a little, though not significantly in both cases. However, the glucosamine released in the medium after 24h of incubation had a significant increment (P⟨0.05) in both cases. The rise of glucosamine in the medium suggests that rumen protozoa have chitinolytic activity. The fact that free amino acids liberated by protozoa in the medium increased (P⟨⟨0.01) in the presence of fungus is a confirmation of their action in the ruminal turnover of fungal protein.

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  • 日本畜産学会報

    日本畜産学会報 64 (6), 584-592, 1993

    公益社団法人 日本畜産学会

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