Developmental Potency of Mouse Embryos Produced by Aggregating the Single Blastomeres of 8-Cell Stage Embryos

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  • マウス8細胞期胚由来の単一割球から作製した集合胚の発生能
  • マウス 8 サイボウキ ハイユライ ノ タンイツ カッキュウ カラ サクセイシ

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Developmental potency of a single blastomere isolated from 8-cell stage embryos obtained from ddY strain mice and of the embryos produced by aggregating two to eight blastomeres was studied. The single blastomeres developed to the morulae slower than the aggregates derived from two blastomeres during culture, but there was no difference between the single blastomeres and the aggregates in the time required for the formation of blastocoele. On the other hand, the development of the aggregates into the morula was slower as the number of blastomeres used for aggregation increased, but there was no difference between the aggregates except the aggregates derived from two blastomeres and the zona free 8-cell embryos in the time required for the development to the early blastocyst. As the number of blastomeres used for aggregation decreased, the increasing rate of embryo size showed to be lower, and the number of cells in the inner cell mass and thet trophoblast was smaller at the blastocyst stage. A single blastomere developed into the blastura which was composed of only trophoblastic cells without inner cell mass. The morula or early blastocyst stage embryos were transferred into the uteri of pseudopregnant females, and the females were autopsied on the 19th day of gestation. Live fetuses were obtained from the aggregates derived from two or more blastomeres, but implantation of the embryos from the single blastomeres did not occur. The results suggest that a single blastomere of 4-cell stage embryo in mouse may has the potency to develop into a normal fetus, whereas a single blastomere of 8-cell stage embryo may not.

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