Identification of genetically modified organisms by detection of target gene pattern using DNA microarrays

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  • DNAマイクロアレイによる遺伝子組換え作物の品種同定検査法

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Abstract

For the identification and quantification of genetically modified organisms (GMOs), one possible simple alternative method relies on the detection of DNA fragments synthesized using random primers without the need for nucleic acid amplification (for example, PCR) on DNA microarrays. Here, we tested simple detection protocols with a DNA microarray, and consequently, we were able to identify five selected GM maize lines by the pattern of spots detected on the DNA microarray. Our protocol requires no specific primers in the target DNA synthesis steps; all target DNA is synthesized by random 9-mer primers in one tube. This study suggests the possibility of detecting transgenes in GMOs and identifying GMO lines by the pattern of independently detected spots, irespective of the position of the target gene sequences in the genomic DNA of each GMO line.

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