凍結融解ウシ除核未受精卵への核移植

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  • Effects of Freezing and Thawing on the Development of Enucleated Bovine Oocytes Following Nuclear Transfer
  • Effects of Freezing and Thawing on the

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The in vitro developmental ability of frozen-thawed enucleated oocytes receiving a donor blastomere of embryos fertilized in vitro was studied. All recipient oocytes were enucleated before freezing and were activated 9 hours before fusion with a donor blastomere from 8-to 20-cell stage embryos. The enucleated oocytes were frozen in 1.0 or 1.5M glycerol by conventional slow cooling methods. The developmental ability of oocytes was slightly higher when they were frozen before rather than after activation (in experiment 1). The developmental ability to 2-cell and 8-cell stage was high (70% and 26%, respectively) when aged oocytes (32 hours of maturation at activation) were used, but the development to blastocyst was only observed in young oocytes (23.5 hours) receiving a donor blastomere (2% in experiment 2). Although the developmental ability to 8-cell stage of frozen-thawed nuclear transferred oocytes was low (15%), a small ratio of them developed to blastocysts (2%).

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