Limiting Amino Acids for Early-Weaned Calves Fed a Corn and Soybean Meal Diet and Response of Plasma Free Amino Acid Concentrations to Abomasally Supplemented Amino Acids

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  • (トウモロコシ+大豆粕)飼料給与時の早期離乳子牛における制限アミノ酸と第四胃内アミノ酸投与に対する血漿遊離アミノ酸濃度の反応
  • Limiting Amino Acids for Early-Weaned C

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Holstein bull calves weaned at 6wk of age were used in two nitrogen (N) balance experiments to identify limiting amino acids for a ration based on corn, soybean meal and rice straw and containing 14.5% CP of DM. The calves were trained to maintain reflex closure of the esophageal groove throughout the experiments that started from 8wk of age. In Experiment 1, three levels (0, 0.022 and 0.111g/kg BW/d) of DL-methionine (DL-Met) were administered through the esophageal groove to 9 calves in a 3×3 Latin square design with three replicates and 10 d- period. In Experiment 2, 18 calves were divided into 3 groups, and allotted to three treatments: abomasal administration of DL-Met (0.111g/kg BW/d), DL-Met with L-tryptophan (L-Trp), or DL-Met with L-Trp and L-Lys monohydrochloride (L-LysHCl) for 2wk each. The amount of supplemental N was adjusted with L-glutamine among treatments in every experiment. As a result, the N balance was improved, and plasma concentrations of branched-chain amino acids (BCAA) were decreased, by the highest dose of DL-Met in Experiment 1. In Experiment 2, however, administration of L-LysHCl and L-Trp in addition to DL-Met improved N balance without decrease in plasma BCAA concentrations.

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