CO₂ anesthesia enhances infection rate of Cordyceps militaris (Hypocreales: Clavicipitaceae) on pupae of the silkworm, Bombyx mori

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  • CO<sub>2</sub> anesthesia enhances infection rate of <i>Cordyceps militaris</i> (Hypocreales: Clavicipitaceae) on pupae of the silkworm, <i>Bombyx mori</i>

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説明

An entomogenous fungus, Cordyceps militaris, grows on larvae and pupae of the silkworm, Bombyx mori. To overcome problems in the establishment of fungal infection caused by advancing pupal-adult development, we tested the effects of carbon dioxide (CO2) anesthesia. Pupae inoculated with C. militaris were held in CO2 anesthesia for 2 days. Almost all pupae exposed to CO2 were infected with C. militaris, but the rate of infection of chilled or untreated pupae decreased with increasing stage of pupal-adult development at inoculation. The results indicate that exposure of silkworm pupae to CO2 enhances infection rate. This treatment will support the mass production of C. militaris for pharmacological uses.<br>

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