Quantitative Analysis of γ-Oryzanol in Foodstuffs by HPLC with Electrochemical and Ultraviolet Detection
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- Okada Yasuyo
- School of Pharmaceutical Science, Mukogawa Women's University
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- Sakai Mai
- School of Pharmaceutical Science, Mukogawa Women's University
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- Shintani Yuko
- School of Pharmaceutical Science, Mukogawa Women's University
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- Semma Masanori
- School of Pharmaceutical Science, Mukogawa Women's University
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- Ito Yoshio
- School of Pharmaceutical Science, Mukogawa Women's University
Bibliographic Information
- Other Title
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- HPLCによる食品中γ-オリザノールの分析法に関する研究
- HPLC ニ ヨル ショクヒン チュウ ガンマ オリザノール ノ ブンセキホウ ニ カンスル ケンキュウ
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Abstract
An analytical method for γ-oryzanol from foodstuffs was studied. γ-Oryzanol from rice bran is a mixture of several ferulate esters, cycroartenyl ferulate and 24-methylenecycloartanyl ferulate are major components. A reverse-phase HPLC method with electrochemical (ECD) and ultraviolet (UV) detection was used for separating the individual components of γ-oryzanol. As the pretreatment, the use of six kinds of solid-extraction cartrides for the extraction of γ-oryzanol was examined. C18 and Florisil types of solid-extraction cartridges were satisfactory for γ-oryzanol extraction from foodstuffs. The recovery of γ-oryzanol (major components) was more than 85 %. ECD was about 6-fold more sensitive than UV.
Journal
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- Japanese Journal of Food Chemistry and Safety
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Japanese Journal of Food Chemistry and Safety 9 (3), 95-100, 2002
Japanese Society of Food Chemistry
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Details 詳細情報について
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- CRID
- 1390001205195342720
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- NII Article ID
- 110007367252
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- NII Book ID
- AA11666400
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- ISSN
- 21896445
- 13412094
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- NDL BIB ID
- 6465319
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed