Determination of gentamicins in meat by LC/FL accompanied with online immunoaffinity chromatography cleanup system

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  • Sakamoto Yasuhiro
    Department of Analytical Chemistry, Faculty of Pharmaceutical Science, Hoshi University
  • Iwasaki Yusuke
    Department of Analytical Chemistry, Faculty of Pharmaceutical Science, Hoshi University
  • Ito Rie
    Department of Analytical Chemistry, Faculty of Pharmaceutical Science, Hoshi University
  • Saito Koichi
    Department of Analytical Chemistry, Faculty of Pharmaceutical Science, Hoshi University

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  • 食肉中アミノグリコシド系抗生物質の分析におけるオンライン - イムノアフィニティークロマトグラフィーシステムの構築

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Abstract

A sample cleanup method that employs immunoaffinity chromatography (IAC) was investigated for potential use in the analysis of residual gentamicins (GMs) in meat samples. A column switching system was constructed to automate IAC operation online. GMs were measured by liquid chromatography with fluorescence detection after derivatization with 9-fluorenylmethylchloroformate. Most of the impurities in the meat samples were eliminated by IAC compared with conventional solid-phase extraction. The average recoveries of GMs from chicken meat and pork meat samples ranged from 77.5% to 89.0% (RSD:<7.0%) and from 75.6% to 87.7% (RSD:<6.4%), respectively. Method detection limit (MDL, S/N=3) and method quantification limit (MQL, S/N>10) were 30 ng/mL and 100 ng/mL, respectively, when calculations were conducted using MDL and MQL of pork meat thigh as total GM concentration. The results suggest that IAC is a useful cleanup method for the determination of residual GMs in meat samples.

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