Molecular Cloning and Expression of cDNAs Encoding Testis-Specific and Non-Specific ATPase Inhibitor-Like Proteins in <i>Bombyx mori</i>
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- Ogura Ichiro
- Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
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- Kusakabe Takahiro
- Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
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- Kawaguchi Yutaka
- Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
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- Maeda Takuji
- Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
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- Koga Katsumi
- Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
Bibliographic Information
- Other Title
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- Molecular Cloning and Expression of cDNAs Encoding Testis-Specific and Non-Specific ATPase Inhibitor-Like Proteins in Bombyx mori
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Description
A cDNA clone encoding an ATPase inhibitor-like protein was found in a Bombyx mori EST (expressed sequence tag) database and designated as BmAl-a. Also a novel cDNA clone encoding a different ATPase inhibitor-like protein was isolated from a testis library of B. mori after mRNA subtraction, and named BmAl-b. Both BmAl-a and BmAl-b cDNA clones were determined for their nucleotide sequences; the deduced amino acid sequences showed that the relevant proteins were composed of 127 and 107 amino acid residues, respectively. The BmAl-a and BmAl-b proteins have the highest homologies of 41% and 37%, respectively, with the Caenorhabditis elegance ATPase inhibitor-like protein CelF, among the animal homologs so far reported. Expression analysis by reverse transcription polymerase chain reaction demonstrated that the BmAl-a mRNA was transcribed in all tissues examined, while the BmAl-b mRNA was expressed exclusively in the testis. A computational analysis of amino acid residues by a method available from a web-server, suggested that BmAl-a is located in the mitochondria, whereas BmAl-b is allocated in organelles other than the mitochondria.
Journal
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- Journal of Insect Biotechnology and Sericology
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Journal of Insect Biotechnology and Sericology 70 (2), 121-128, 2001
The Japanese Society of Sericultural Science
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Details 詳細情報について
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- CRID
- 1390001205196018944
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- NII Article ID
- 130003852098
- 10010586593
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- NII Book ID
- AA11558849
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- ISSN
- 18847978
- 13468073
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- NDL BIB ID
- 5952224
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed