書誌事項
- タイトル別名
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- Effects of Glucose and Amino Acids on In-Vitro Development of Rat Preimplantation Embryos, and Morphological Features of the Developed Blastocysts
- Effects of Glucose and Amino Acids on I
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This study examined the effects of glucose and amino acids on in vitro development of Sprague-Dawley (SD) rat 1-cell embryos, and the morphological features of the developed blastocysts. Approximately 60% of one-cell embryos developed in vitro to the blastocyst stage when 0.5mM glucose was added to a chemically defined medium (modified hamster embryo culture medium-1; HECM-1), and glucose and amino acids had synergistic effects on the development. However, the mean cell number of the blastocysts developed in vitro from 1-cell stage embryos was significantly less compared with that of the blastocysts developed in vivo (P<0.01). The morphological features of 1-cell embryos developed in vitro to the blastocyst stage differed somewhat to those of the in vivo-developed blastocysts; less development of blastocoele, narrow intercellular space in inner cell mass and high electron-dense mitochondria. These results suggest that SD rat 1-cell embryos can developed in vitro to the blastocysts stage, and glucose and amino acids have important roles in the embryonic development. However, the blastocysts developed in vitro from 1-cell embryos may have some differences in comparison with the blastocysts developed in vivo.
収録刊行物
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- 日本畜産学会報
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日本畜産学会報 69 (7), 637-645, 1998
公益社団法人 日本畜産学会
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詳細情報 詳細情報について
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- CRID
- 1390001205196052096
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- NII論文ID
- 130000743078
- 10021725569
- 10020394794
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- NII書誌ID
- AN00195188
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- ISSN
- 18808255
- 00215309
- 1346907X
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- NDL書誌ID
- 4536236
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可