Isolation and Characterization of Citrate Metabolism-Deficient Mutants in Lactobacillus plantarum IFO 3070

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  • Lactobacillus plantarum IFO 3070におけるクエン酸代謝機能欠損株の単離とその諸性状
  • Lactobacillus plantarum IFO 3070 ニ オケル

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Abstract

The non-citrate-fermenting(Cit-) mutants of Lactobacillus plantarum strain IFO 3070 were isolated and examined for their genetic and enzymatic characteristics. After their growth in broth containing either acridine orange or ethidium bromide, each culture was plated on the citrate-fermenting agar supplemented with potassium ferricyanide and ferric citrate. After the treatment with acridine orange, 1.9% of cells developed white colonies which meaned the phenotype of Cit-. On the other hand, treatment with ethidium bromide resulted in the formation of white colonies in 12.2% of cells. Two Cit- mutants, the strain 3070-5 and 3070-11, obtained from each plate, were unable to produce diacetyl and acetoin from citrate. The agarose gel electrophoretic analysis of plasmid DNA from the strain 3070-5 and 3070-11 indicated that these mutants were missing a plasmid with a molecular weight of approximately 7 Mdal. One representative Cit- mutant(3070-5) from among these was further examined for its citrate-transporting and citrate-splitting activity. Although the strain 3070-5 was unable to transport citrate into the cell, it possessed the citrate-splitting activity. These results suggest that the citrate-fermenting ability of Lb. plantarum strain IFO 3070 may be linked with a 7 Mdal plasmid, which associate with the citrate permease production.

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