Studies on the Recovery of Genomic DNA and Functional Genes from Mammalian Pelt Specimens

DOI
  • ASANO Yumi
    Graduate School of Agriculture, Nippon University Graduate School of Agriculture and Life Science, The University of Tokyo
  • UEKITA Takamasa
    Graduate School of Agriculture and Life Science, The University of Tokyo
  • KASAI Kyoko
    Graduate School of Agriculture and Life Science, The University of Tokyo
  • ENDO Hideki
    National Science Museum
  • YAMADA Tadasu K.
    National Science Museum
  • SABURI Sakura
    Graduate School of Agriculture and Life Science, The University of Tokyo The Burnham Institute
  • YAMANOUCHI Keitaro
    Graduate School of Agriculture and Life Science, The University of Tokyo
  • TOJO Hideaki
    Graduate School of Agriculture and Life Science, The University of Tokyo
  • NATORI Masahiko
    Graduate School of Agriculture, Nippon University
  • TACHI Chikashi
    School of Veterinary Medicine, Azabu University

Bibliographic Information

Other Title
  • 哺乳類の毛皮標本からのDNA抽出ならびに機能遺伝子の回収に関する研究

Abstract

To recover functional genes from specimens of extinct or endangered species is of prime importance from the view point of conservation of genetic resources as well as that of evolutional studies of genes. Hides from stuffed specimens or pelts are expected to serve as suitable materials for the purpose. However, methods have to be developed so that the damages to the original precious specimens will be minimal. Methods so far described in the literature requires relatively large amounts of the specimens and not suitable for the analysis of highly valuable specimens. In the present paper, an improved method is described to extract genomic DNA from the hide or pelt specimens of approximately 1mm2. Parameters and conditions optimal for PCR analysis of the extracted DNA are also investigated. Employing the procedures we established, genomic DNA was extracted from various carnivoran hide specimens with different passage of time after their preparation, and the partial DNA sequences of the genes coding for amelogenin, a major protein component of enamel of the teeth, were determined.

Journal

Keywords

Details 詳細情報について

  • CRID
    1390001205196449152
  • NII Article ID
    130000742936
  • DOI
    10.2508/chikusan.70.10_351
  • ISSN
    18808255
    1346907X
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

Report a problem

Back to top