Hyperactivated Motility is Induced by Reagents Depressing the Function of Calcium in Mouse Sperm
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- AOKI Fugaku
- Graduate School of Agriculture and Life Sciences, University of Tokyo
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- SAKAI Senkiti
- Graduate School of Agriculture and Life Sciences, University of Tokyo
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- KOHMOTO Kaoru
- Graduate School of Agriculture and Life Sciences, University of Tokyo Faculty of Veterinary and Animal Science, Nihon Veterinary and Animal Science University
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To investigate the role of Ca2+ on the regulatory mechanism of hyperactivation, mouse sperm were treated with a calcium ionophore, ionomycin and a calmodulin inhibitor, W-13, and were analyzed for swimming pattern and flagellar bending. On the successive frames of photographs, the linearity of swimming and the bend angle were measured. Both reagents induced a non-linear swimming pattern as is observed in spontaneously hyperactivated sperm. However, changes in flagellar bending occurred in a different manner. After the occurrence of hyperactivation, the bend angle in the midpiece region was increased to the same direction as the curve of hook-shaped heads. Treatment with W-13 or ionomycin plus EGTA also increased the bend angle to that direction, whereas ionomycin plus Ca2+ increased it to opposite direction. The analysis for asymmetry of flagellar bending also revealed that the treatment with W-13 increased asymmetry to the same direction as the curve of head as is observed in spontaneously hyperactivated sperm and that ionomycin plus Ca2+ increased it to opposite direction. These results suggest that the depression of function of machinery controlled by Ca2+ is involved in the mechanism by which the sperm begin hyperactivated motility.
収録刊行物
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- 日本畜産学会報
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日本畜産学会報 70 (2), 53-60, 1999
公益社団法人 日本畜産学会
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詳細情報 詳細情報について
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- CRID
- 1390001205196632064
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- NII論文ID
- 130000742897
- 10020393341
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- NII書誌ID
- AN00195188
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- ISSN
- 18808255
- 00215309
- 1346907X
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- NDL書誌ID
- 4687800
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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