The distribution of H+-translocating vacuolar-type ATPase in the middle silk gland cell of Bombyx mori
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- Azuma Masaaki
- Laboratory of Insect Biochemistry, Faculty of Agriculture, Tottori University
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- Miyamoto Yasunori
- Laboratory of Insect Biochemistry, Faculty of Agriculture, Tottori University
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- An Zhigang
- Laboratory of Insect Biochemistry, Faculty of Agriculture, Tottori University
書誌事項
- タイトル別名
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- The Distribution of H<sup>+</sup>-Translocating Vacuolar-Type ATPase in the Middle Silk Gland Cell of <i>Bombyx mori</i>
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説明
A proton-translocating vacuolar-type ATPase (V-ATPase) was identified and characterized in the middle silk gland (MSG) of Bombyx mori. The presence of V-ATPase in a microsomal fraction was verified by immunoblots using an antiserum to the V-ATPase holoenzyme from Manduca sexta midgut and by measuring the enzyme activity as an azide- and vanadate-insensitive, bafilomycin-sensitive ATPase. The antiserum localized the V-ATPase at the apical cell surface of the posterior division of MSG, which is characterized as unusual huge vacuoles, cavities and numerous pits. The strong immunoreactions of V-ATPase were also distributed at the periphery of these structures, which appeared to be filled with the secreted sericin. These observations suggest that the plasma membrane V-ATPase in the posterior division of MSG seems to be important for the active exocytosis and secretion of sericin to establish the electrochemical proton gradients by pumping the proton out of the cell towards the glandular lumen.
収録刊行物
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- Ｊｏｕｒｎａｌ ｏｆ Ｉｎｓｅｃｔ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ ａｎｄ Ｓｅｒｉｃｏｌｏｇｙ
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Ｊｏｕｒｎａｌ ｏｆ Ｉｎｓｅｃｔ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ ａｎｄ Ｓｅｒｉｃｏｌｏｇｙ 70 (1), 25-32, 2001
社団法人 日本蚕糸学会
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詳細情報 詳細情報について
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- CRID
- 1390001205196711680
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- NII論文ID
- 130003706278
- 10010586090
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- NII書誌ID
- AA11558849
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- ISSN
- 18847978
- 13468073
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- NDL書誌ID
- 5806520
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
