Spatial and temporal changes of mitotic activity in the epidermis during larval development of the silkworm, <i>Bombyx mori</i>

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  • Shimura Sachiko
    Invertebrate Gene Function Research Unit, National Institute of Agrobiological Sciences, Tsukuba
  • Kiuchi Makoto
    Invertebrate Gene Function Research Unit, National Institute of Agrobiological Sciences, Tsukuba
  • Kiguchi Kenji
    Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

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  • Spatial and temporal changes of mitotic activity in the epidermis during larval development of the silkworm, Bombyx mori

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Abstract

The spatial and temporal characteristics of cell divisions in the dorsal epidermis of the 5th segment of the silkworm, Bombyx mori, were investigated from the 3rd instar to the wandering stage. Whole-mount immunohistochemistry using an antibody specific for M phase nuclei was used to examine the mitotic characteristics, and changes in cell size and ploidy level were also examined. During the 3rd and 4th instar stages, mitoses occurred during mid-feeding stage, but not in the early and late feeding stages and molting stage. By contrast, in the final 5th instar mitoses occurred during the period from the late feeding stage to the wandering stage. The spatial characteristics of mitotic cell division were also stage specific. Mitoses occurred in the whole segment in the 3rd instar, whereas in the 4th and 5th instars mitotic figures were observed primarily in the anterior half of the segment, with few in the posterior half. Cell size and ploidy level appeared to correlate with cell division, resulting in a clear boundary along the transverse middle line of the larval segment. These results suggest that there are three types of mitotic classes in the epidermis during larval development in the silkworm, namely early instar-specific, penultimate instar-specific and final instar-specific.<br>

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