Preliminary Examination on the Intragastric Continuous Infusion of Volatile Fatty Acid Triglyceride and Casein Solutions in Sheep

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  • めん羊における揮発性脂肪酸トリグリセライドおよびカゼイン連続注入による人工栄養試験の予備的検討
  • メンヨウ ニ オケル キハツセイ シボウサン トリグリセライド オヨビ カゼイ

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Abstract

The total infusion of nutrients was examined in sheep via the technique of continuous alimentation. Four abult Suffolk sheep fitted with a permanent ruminal cannula and a simple Tshaped duodenal cannula were used. A peristaltic pump was used to infuse solutions of volatile fatty acid triglycerides (VFA-TG) consisting of 70% triacetin, 20% tripropionin, 10% tributyrin on the basis of energy and minerals into the rumen, and casein solution into the duodenum. Each experimental period consisted of a common feeding period (non-infusion period) of 14 days when orchardgrass hay and concentrate ration was offered, and a changeover and adaptation period of 5 days followed by an infusion period of 14 days. The total daily amounts of energy and protein infused were equivalent to 1.5 and 1.2 times the maintenance amount, respectively. Ruminal and blood samples were withdrawn through the ruminal cannula and from a jugular vein catheter in each period. In the latter half of each of the non-infusion and infusion periods, glucosetreatment capacity was determined by the glucose load experiment, and insulin secretory response of glucose and the responsiveness of peripheral tissues to insulin were determined by the hyperinsulinemic euglycemic clamp (EGC) technique. Infusion of VFA-TG into the rumen caused a marked increase in ruminal TG concentration and a slight decline in ruminal pH values during the latter half of the infusion period. Intragastric infusion of nutrients resulted in slightly lower plasma ammonia and urea concentrations in comparison with non-infusion period. There was no significant effect of the infusion on plasma glucose, insulin, non-esterified fatty acid or triglyceride concentrations. Plasma lactic acid concentration was higher in the infusion period than in the non-infusion period (p<0.01). In the glucose load experiment, glucose treatment capacity and insulinogenic index (IGI) represented as a ratio of insulin increment to glucose increment were not significantly affected by the intragastric infusion of nutrients. In the EGC experiment, tissue responsiveness to insulin was slighty high in the infusion period. Although blood pH, Pco2, HCO3- and base excess tended to be higher during the latter half of the infusion period, sheep had no abnormal condition from a general clinical view. These findings may indicate that the technique of continuous infusion of total nutrients in the present experiment maintained sheep in a normal physiological situation, as in a common feeding, over a 2 week infusion period.

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