A New Screening Method for Probiotic Lactic Acid Bacteria in the Lactobacillus acidophilus Group by Using Carbohydrate Probes
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- SAITO Tadao
- Graduate School of Agricultural Science, Tohoku University
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- MATSUMURA Atsushi
- Graduate School of Agricultural Science, Tohoku University
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- KITAZAWA Haruki
- Graduate School of Agricultural Science, Tohoku University
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- KAWAI Yasushi
- Graduate School of Agricultural Science, Tohoku University
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- ITOH Takatoshi
- Graduate School of Agricultural Science, Tohoku University
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- HASHIBA Honoh
- Technical Research Institute, Snow Brand Milk Products Co., Ltd
書誌事項
- タイトル別名
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- New Screening Method for Probiotic Lactic Acid Bacteria in the Lactobacillus acidophilus Group by Using Carbohydrate Probes
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抄録
To select probiotic strains of the Lactobacillus (L.) acidophilus group with high adhesion to human intestinal tracts for developing the functional yogurt, a new screening method using biotinylated carbohydrate probe (BCP) was introduced. After incubation of living cells of 30 strains of the L. acidophilus group with 5 kinds of BCPs which possess representative partial structures of the sugar chains constituting of human colonic mucin, the interaction between cell-surface lectins and BCP were evaluated. The highest reactivity was detected in 19 strains (about 63%) with a trisaccharide probe (GalNAcα (1→3)[Fucα (1→2)] Galβ 1→). The cell-surface lectin activity was first clearly confirmed in the B group of the L. acidophilus group. The adhesion evaluation score (AE score, total sum of each absorbance of 5 BCPs samples) is considered to be useful as an indicator for selection of the probiotic strains which have high adhesiveness in human gastrointestinal tract.
収録刊行物
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- 日本畜産学会報
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日本畜産学会報 71 (1), 103-108, 2000
公益社団法人 日本畜産学会
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詳細情報 詳細情報について
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- CRID
- 1390001205196997248
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- NII論文ID
- 10021722226
- 10020391451
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- NII書誌ID
- AN00195188
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- ISSN
- 18808255
- 00215309
- 1346907X
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- NDL書誌ID
- 4970300
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 使用不可