Transient expression assay reveals kinetic difference in the proteolytic processing between Dronc proteins from the gypsy moth <i>Lymantria dispar</i> and the silkworm <i>Bombyx mori</i>

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  • Kitaguchi Koji
    Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University
  • Hamajima Rina
    Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University
  • Yamada Hayato
    Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University
  • Kobayashi Michihiro
    Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University
  • Ikeda Motoko
    Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University

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  • Transient expression assay reveals kinetic difference in the proteolytic processing between Dronc proteins from the gypsy moth Lymantria dispar and the silkworm Bombyx mori

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Abstract

Ld652Y cells from the gypsy moth, Lymantria dispar, are highly sensitive to apoptotic stimuli compared to BM-N cells from the silkworm, Bombyx mori, and undergo apoptosis upon infection with various nucleopolyhedroviruses (NPVs). We previously cloned and characterized Dronc homologues from L. dispar Ld652Y and B. mori BM-N cells and demonstrated that L. dispar Dronc (Ld-Dronc) undergoes proteolytic processing more rapidly and extensively than does B. mori Dronc (Bm-Dronc) upon infection with vAcΔp35, a p35-defective Autographa californica multiple NPV. Here, we comparatively examined expression and proteolytic processing of the Ld- and Bm-Dronc proteins in the transient expression assays using three different insect cell lines, Ld652Y, BM-N and Spodoptera frugiperda Sf9. We demonstrate that even in the transient expression assays, Ld-Dronc undergoes proteolytic processing and activates caspase-3-like protease more rapidly and extensively than Bm-Dronc in a cell-line independent manner. These results indicate that intrinsic properties of Dronc are involved in the differing capacity of Ld652Y and BM-N cells to induce apoptosis in response to apoptotic stimuli, including NPV infection.<br>

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