Flow cytometric analysis of various organs and cytochimeras of mulberry (Morus spp.)
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- Yamanouchi Hiroaki
- Institute of Radiation Breeding, National Institute of Agrobiological Sciences
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- Koyama Akio
- Genebank, National Institute of Agrobiological Sciences
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- Takyu Toshio
- Institute of Radiation Breeding, National Institute of Agrobiological Sciences
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- Yoshioka Terutaka
- Institute of Radiation Breeding, National Institute of Agrobiological Sciences
書誌事項
- タイトル別名
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- Flow cytometric analysis of various organs and cytochimeras of mulberry (<i>Morus</i> spp.)
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説明
Flow cytometry is a useful technique for studying plant genome size and ploidy and for analyzing the degree of polysomaty of various organs. Polysomaty is a common biological phenomenon in higher plants and is detectable as multiple peaks in the histogram by flow cytometric analysis. However, if polysomaty occurs in the target tissue, the multiple peaks may cause mis-estimates of genome size and ploidy obtained by flow cytometric analysis. In this study, we used flow cytometry to determine whether polysomaty occurred in various organs of several mulberry species. We did not find markedly polysomatic organs in any of the above-ground vegetative organs, whereas anthers exhibited a polysomatic state. Based on these results, we tried to ascertain the possibility distinguishing between two types of periclinal cytochimeras by flow cytometric analysis of mulberry leaves. One type of the cytochimera was distinguishable from its progenitor and the other type of cytochimera by flow cytometry. <br>
収録刊行物
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- Journal of Insect Biotechnology and Sericology
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Journal of Insect Biotechnology and Sericology 77 (2), 2_95-2_108, 2008
社団法人 日本蚕糸学会
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詳細情報 詳細情報について
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- CRID
- 1390001205197206400
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- NII論文ID
- 10021221136
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- NII書誌ID
- AA11558849
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- ISSN
- 18847978
- 13468073
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- NDL書誌ID
- 9574024
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可