Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences
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- Kawanishi Yuichi
- Functional Genomics Group, Center of Molecular Biosciences (COMB), University of the Ryukyus
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- Banno Yutaka
- Institute of Genetic Resources, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
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- Fujimoto Hirofumi
- Division of Radiological Protection and Biology, National Institute of Infectious Diseases
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- Kab Nho Si
- College of Agriculture and Life Sciences, Kyungpook National University
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- Tu Zhenli
- College of Animal Sciences, Zhejiang University
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- Mita Kazuei
- Division of Insect Science, National Institute of Agrobiological Sciences
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- Tsuchida Kozo
- Division of Radiological Protection and Biology, National Institute of Infectious Diseases
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- Takada Naoko
- Division of Radiological Protection and Biology, National Institute of Infectious Diseases
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- Maekawa Hideaki
- Functional Genomics Group, Center of Molecular Biosciences (COMB), University of the Ryukyus Division of Radiological Protection and Biology, National Institute of Infectious Diseases
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- Nakajima Yumiko
- Functional Genomics Group, Center of Molecular Biosciences (COMB), University of the Ryukyus
書誌事項
- タイトル別名
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- Method for rapid distinction of <i>Bombyx mandarina</i> (Japan) from <i>B. mandarina</i> (China) based on rDNA sequence differences
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説明
The EcoRI site is located between the 5.8S and 28S rRNA genes in Bombyx mandarina (Japan)(Japanese type rDNA) but is absent in the rDNA of B. mandarina (China)(B. mori type rDNA) including the domesticated B. mori. Digestion of PCR products amplified by primers of both flanking sides of this EcoRI site with EcoRI should only be successful for amplification products of B. mandarina (Japan) containing Japanese type rDNA. The distribution of Japanese type rDNA in Japan, Korea, and China was determined using this rapid detection method. Japanese type was not detected in China or in Korea despite having 27 chromosomes. These findings suggest that the choromosome fusion and the rDNA type separation were independent evolutionary events. Invasion of B. mori chromosome #11 into Japanese B. mandarina (Japan) was examined.<br>
収録刊行物
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- Journal of Insect Biotechnology and Sericology
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Journal of Insect Biotechnology and Sericology 77 (2), 2_79-2_85, 2008
社団法人 日本蚕糸学会
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詳細情報 詳細情報について
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- CRID
- 1390001205197208960
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- NII論文ID
- 10021221089
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- NII書誌ID
- AA11558849
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- ISSN
- 18847978
- 13468073
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- NDL書誌ID
- 9573995
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可