Construction and Analysis of Subtractive cDNA Library of Muscular Tissue in Hybrid of Chicken-Quail Related to Myogenesis
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- Liang YaoWei
- Animal Genetics and Breeding Department, Animal Science and Technology College, Shihezi University, China
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- Zhen Wei
- Animal Genetics and Breeding Department, Animal Science and Technology College, Shihezi University, China
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- G.L. Jiaerheng MiLa
- Animal Genetics and Breeding Department, Animal Science and Technology College, Shihezi University, China
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- Zhao ZongSheng
- Animal Genetics and Breeding Department, Animal Science and Technology College, Shihezi University, China
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- Zhang HongMei
- Analysis Department, The First Affiliated Hospital of Medical Colledge of Shihezi University, China
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Abstract
Myogenesis is a complex developmental progress in which a variety of transcription factors play essential roles in regulating myogenesis. However, the genetics programs that control myogenesis molecular mechanism are poorly understood. Therefore, meat-breeding and egg-breeding chicken were selected as male parent, Korean quails were used as female parent for hybridizing. To identify differentially expressed genes between the different hybrids, suppression subtractive hybridization (SSH) have been undertaken for generating cDNA collections of representative mRNAs specific to muscle tissue with meat-hybrid versus egg-hybrid. Following SSH, 54 clones were sequenced and analysed through BLASTX. The results showed that 26 Expressed Sequence Tags (ESTs) found no homology while the other 22 ESTs found the homology in GenBank and 4 ESTs might have correlation with myogenesis.
Journal
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- The Journal of Poultry Science
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The Journal of Poultry Science 50 (4), 326-331, 2013
Japan Poultry Science Association
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Keywords
Details 詳細情報について
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- CRID
- 1390001205205977472
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- NII Article ID
- 130004446404
- 40019836685
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- NII Book ID
- AA11564513
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- COI
- 1:CAS:528:DC%2BC3sXhvVCls7%2FI
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- ISSN
- 13490486
- 13467395
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- NDL BIB ID
- 024947610
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed