Effects of Egg Storage Position and Injection of Solutions in Stored Eggs on Hatchability in Chickens (Gallus domesticus)

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  • Tiwary Anil Kr.
    Graduate School for International Development and Cooperation, Hiroshima University
  • Maeda Teruo
    Graduate School of Biosphere Science, Hiroshima University

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These experiments were conducted to investigate the effects of egg storage position and injection of solutions into stored eggs on hatchability in chickens. In experiment 1, a total of 689 eggs were randomly divided into two groups (Normal position : eggs with small end down, Opposite position : eggs with small end up) and were stored at 15°C and 60% relative humidity (RH) for one, two or three weeks in one of the two positions. Eggs were then incubated at 37.5°C and 60% RH. In experiment 2, a total of 120 eggs were divided into two groups (Rotation : the egg box was rotated 45° on the long axis twice a day, Non-rotation : eggs were stored without movement). These eggs were stored for 3 or 4 weeks then incubated as in experiment 1. In experiment 3, a total of 240 eggs were divided randomly into 4 groups (Control : no treatment, Needle : pricked with a needle, Saline : injected with physiological saline, MEM : injected with MEM solution). After storing at 15°C and 60% RH for 2 or 3 weeks, eggs were injected with different solutions and then incubated at 37.5°C and 60% RH. In experiment 1, egg weight loss% differed significantly (P<0.01) within the storage period but no difference due to the storage position was observed. Hatchability was significantly higher (p<0.05) in eggs stored for 2 or 3 weeks in the opposite position. Early mortality was the highest after 3 weeks for the normal position group. In experiment 2, rotation had no significant effect on hatchability. There was a sharp decline in hatchability when eggs were stored for 4 weeks. In experiment 3, injection of physiological saline and MEM solutions did not improve hatchability after 3 or 4 weeks of storage.

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