Chicken Ovotransferrin Variants OTF<sup>B</sup> and OTF<sup>C</sup> Harboring Substitution of GAT (Asp) to AAT (Asn) in the Codon 500 and their Antimicrobial Activity
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- Kinoshita Keiji
- Avian Bioscience Research Center, Graduate School of Bioagricultural Sciences, Nagoya University, Japan
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- Myint Si Lhyam
- United-graduate School of Agriculture, Kagoshima University, Japan
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- Shimogiri Takeshi
- Faculty of Agriculture, Kagoshima University, Japan
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- Ibrahim Hisham R.
- Faculty of Agriculture, Kagoshima University, Japan
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- Kawabe Kotaro
- Natural Science Centre for Research and Education, Kagoshima University, Japan
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- Okamoto Shin
- Faculty of Agriculture, Kagoshima University, Japan
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- Lee Yen-Pei
- Faculty of Animal Science, National Chung Hsing University, Taiwan
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- Matsuda Yoichi
- Avian Bioscience Research Center, Graduate School of Bioagricultural Sciences, Nagoya University, Japan
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- Maeda Yoshizane
- Faculty of Agriculture, Kagoshima University, Japan
Bibliographic Information
- Other Title
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- Chicken Ovotransferrin Variants OTF[B] and OTF[C] Harboring Substitution of GAT (Asp) to AAT (Asn) in the Codon 500 and their Antimicrobial Activity
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Abstract
<p>Chicken egg white ovotransferrin (OTF) has been reported to exist in three electrophoretic variants (OTFA, OTFB and OTFC). In this report, we identified a causal polymorphism between the OTFB and OTFC variants in Japanese and Taiwanese native chickens and compared the antibacterial activity between these two variants. The cDNA sequence analyses from Satsumadori oviducts revealed that three non-synonymous SNPs T1809G (Ser52Ala), A2258G (Ile96Val) and G7823A (Asp500Asn) corresponded to the OTF electrophoretic phenotypes. Of the three SNPs, the G7823A mutation perfectly corresponded to the electrophoretic phenotypes OTFB (G/G, Asp500Asp), OTFB/C (G/A, Asp500Asn) and OTFC (A/A, Asn500Asn) in three chicken populations. The variants OTFB and OTFC exhibited similar antibacterial potency against Gram-positive and Gram-negative bacteria. This study provides, for the first time, molecular information on polymorphism of OTFB and OTFC variants of chicken ovotransferrin and its effect on the antimicrobial activity of the respective variants.</p>
Journal
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- The Journal of Poultry Science
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The Journal of Poultry Science 53 (4), 257-263, 2016
Japan Poultry Science Association
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Details 詳細情報について
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- CRID
- 1390001205208156032
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- NII Article ID
- 130005433120
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- NII Book ID
- AA11564513
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- ISSN
- 13490486
- 13467395
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- NDL BIB ID
- 027670116
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed