Clonal Propagation System Using Plant Tissue Culture and ex vitro Rooting in Japanese Wild Vaccinium and Blueberry Cultivars
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- Sato-Yamauchi Makiko
- Faculty of Agriculture, University of Miyazaki
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- Tsuda Hirotoshi
- Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki
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- Araki Keisuke
- Faculty of Agriculture, University of Miyazaki
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- Uchida Asuka
- Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki
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- Yasuda Kiichi
- Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki
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- Tetsumura Takuya
- Faculty of Agriculture, University of Miyazaki
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- Komatsu Haruki
- School of Agriculture, Tokai University
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- Kunitake Hisato
- Faculty of Agriculture, University of Miyazaki
Bibliographic Information
- Other Title
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- 我が国自生のスノキ属植物とブルーベリー栽培品種における植物組織培養と試験管外発根を利用したクローン増殖
- ワガクニ ジセイ ノ スノキゾク ショクブツ ト ブルーベリー サイバイ ヒンシュ ニ オケル ショクブツ ソシキ バイヨウ ト シケンカン ガイ ハッコン オ リヨウ シタ クローン ゾウショク
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Description
In vitro shoot proliferation and ex vitro rooting of Japanese wild Vaccinium species and blueberry cultivars were investigated. Multiple shoot induction and proliferation were highest in medium with 5 mg·L−1 zeatin and multiple shoots were obtained with a 5.6–40.6% induction rate. The most efficient proliferation was obtained by subculturing multiple shoots at 2–3-month intervals. Rooting occurred early when the plants were treated with indole-3-butyric acid (IBA), and the rooting rate increased by raising the IBA concentration. The light condition had no effect on IBA-treated shoots. The rooting rate ex vitro was higher than in vitro, although cultivar and species differences were seen. There was a rooting rate of 48% or more in Natsuhaze (Vaccinium oldhamii Miq.) after one month of acclimatization, and a very high rooting rate of 72% was shown in shoots 3 cm in length. Ex vitro rooting using mist irrigation in a glasshouse was attempted with the aim of practical implementation. The survival rate after two months of acclimatization was 95.8, 75.0 and 91.7% in Natsuhaze, ‘Berkeley’, and ‘Redpearl’, and the rooting rate was 80.6, 63.9 and 83.3%, respectively. Within 12 months, these plants were 20 to 30 cm tall and suitable for field planting.<br>
Journal
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- Horticultural Research (Japan)
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Horticultural Research (Japan) 11 (1), 13-19, 2012
THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
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Details 詳細情報について
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- CRID
- 1390001205208875264
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- NII Article ID
- 130004938720
- 40019188535
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- NII Book ID
- AA11608561
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- ISSN
- 18803571
- 13472658
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- NDL BIB ID
- 023502381
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL Search
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed