Low Polyphenol Oxidase Mutant Induced by <sup>12</sup>C<sup>6+</sup> Ion Beam Irradiation to Protoplasts of Lettuce (<i>Lactuca sativa</i> L.)

DOI JASI Web Site Web Site 1 Citations 15 References
  • Sawada Rimpei
    Kumamoto Prefectural Agricultural Research Center Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
  • Hirano Tomonari
    Nishina Center for Accelerator-Based Science, RIKEN Faculty of Agriculture, Miyazaki University
  • Iimure Kazuhiko
    Kumamoto Prefectural Agricultural Research Center
  • Abe Tomoko
    Nishina Center for Accelerator-Based Science, RIKEN
  • Ozaki Yukio
    Faculty of Agriculture, Kyushu University

Bibliographic Information

Other Title
  • レタス（<i>Lactuca sativa</i> L.）プロトプラストへのイオンビーム照射によって出現した低ポリフェノールオキシダーゼ活性を示す突然変異体
  • レタス(Luctuca sativa L.)プロトプラストへのイオンビーム照射によって出現した低ポリフェノールオキシダーゼ活性を示す突然変異体
  • レタス(Luctuca sativa L.)プロトプラスト エ ノ イオンビーム ショウシャ ニ ヨッテ シュツゲン シタ テイポリフェノールオキシダーゼ カッセイ オ シメス トツゼン ヘンイタイ

Search this article

Abstract

<p>The induction of polyphenol oxidase (PPO) gene mutation through ion beam irradiation of protoplasts was attempted for the production of lettuce plants with a low browning property as a cut vegetable. It was clarified that the optimum protoplast density was 0.63–0.75 × 104 mL–1 for shoot regeneration from protoplasts of lettuce ‘Round’. Effects of 12C6+ ion beam irradiation on the growth of protoplasts were investigated, and the percentage of colony formation decreased as the irradiation dose increased. The dose leading to 50% cell growth as compared with the untreated control was approximately 3.3 Gy. Two out of 869 calli irradiated with 12C6+ ion beams were found to be PPO gene mutations by the TILLING method. PPO activities of the regenerated plants from the two mutant calli named 2Gy-3-36 and 5Gy-2-27 were preliminarily assayed, and lettuce plants from 2Gy-3-36 exhibited half of the PPO activity as compared with the non-irradiated controls. A part of the M2 population from 2Gy-2-36 exhibited the property of no band of heteroduplex cleavage by Cel I, with markedly low PPO activity as the control.</p>

Journal

Citations (1)*help

See more

References(15)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top