DNA Markers Linked to a Recessive Gene Controlling Single Flower Type Derived from a Wild Species, Dianthus capitatus ssp. andrzejowskianus

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  • Onozaki Takashi
    National Institute of Floricultural Science, National Agriculture and Food Research Organization
  • Yoshinari Tsutomu
    Tochigi Prefectural Agricultural Experiment Station
  • Yoshimura Tadahisa
    Miyagi Prefectural Agriculture and Horticulture Research Center
  • Yagi Masafumi
    National Institute of Floricultural Science, National Agriculture and Food Research Organization
  • Yoshioka Satoshi
    National Institute of Floricultural Science, National Agriculture and Food Research Organization
  • Taneya Mitsuyasu
    Chiba Prefecture Agriculture Research Center, Southern Prefectural Horticulture Institute
  • Shibata Michio
    National Institute of Floricultural Science, National Agriculture and Food Research Organization

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Other Title
  • ダイアンサス属野生種Ｄｉａｎｔｈｕｓ　ｃａｐｉｔａｔｕｓ　ｓｓｐ．　ａｎｄｒｚｅｊｏｗｓｋｉａｎｕｓ由来の劣性一重咲き遺伝子に連鎖したＤＮＡマーカー
  • ダイアンサスゾク ヤセイシュ Dianthus capitatus ssp andrzejowskianus ユライ ノ レッセイ ヒトエ ザキ イデンシ ニ レンサ シタ DNA マーカー

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Abstract

Flower type of single or double is one of the important characters in carnation (Dianthus caryophyllus L.). Therefore, we selected random amplified polymorphic DNA (RAPD) markers associated with the genes controlling flower type in a segregated population of 127 progeny plants derived from a cross between ‘Carnation Nou No. 1’ (double) and ‘Pretty Favvare’ (single), which were used for construction of our genetic linkage map in carnations. Four RAPD markers identified by bulked segregant analysis of 696 primers were linked to a recessive gene controlling a single flower type derived from a wild species, D. capitatus ssp. andrzejowskianus. In particular, three RAPD markers, OM19-800, AT90-1000, DT52-700 appeared in all 45 single plants individually, but not in any of the double plants, indicating these three markers were tightly linked to the gene controlling the single flower type. The RAPD marker AT90-1000 was successfully converted to a sequence-tagged site (STS) marker. Linkage analysis revealed that this single gene was located on group 16 in our genetic linkage map. However, the STS marker was not detected in the four single flowered carnation cultivars. This result indicates that this STS marker is highly specific for the single gene derived from D. capitatus ssp. andrzejowskianus.<br>

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