Protein Kinase C as a Biomarker for Assessing the Effect of Environmental Stress and Fungal Invasion on Plant Defense Mechanism

DOI IR NDL Digital Collections JASI Web Site View 1 Remaining Hide 1 Citations 22 References
  • KIRIBUCHI Kyoko
    Graduate School of Science and Engineering, Saitama University
  • DUNLAP Debra Y.
    Department of Environmental Toxicology and Center for Environmental Health Research, University of California
  • MATSUMURA Fumio
    Department of Environmental Toxicology and Center for Environmental Health Research, University of California
  • YAMAGUCHI Isamu
    The Institute of Physical and Chemical Research (RIKEN)

Bibliographic Information

Other Title
  • 植物の環境ストレスと病原菌感染における防御機構のバイオマーカーとしてのプロテインキナーゼC
  • Protein Kinase C as a Biomarker for Ass

Search this article

Abstract

Plants are known to activate specific defense mechanisms in response to invasion by pathogens and environmental stresses. We examined the possibility of utilizing the changes in the amounts of protein kinase C (PKC) and its associated components of this signal transduction pathway as a biomarker of exposure of plants to the stress factors, using young rice plants as a model and a Western blotting method as the experimental tool. Preliminary studies have shown that PKC is a consistently more sensitive marker of exposure to a variety of environmental stress factors as compared to phospholipase C (PLC) or G-protein. The titer of PKC increased as a result of exposure to herbicides, low concentrations of copper, fungicides and other chemicals. The same trend was observed when rice plants were stressed by severe physical treatment such as broken stems, deprivation of sunlight and transplantation. On the other hand, PKC levels decreased upon exposure to high winds and high concentration of copper. The most drastic rise in PKC was observed when rice plants were inoculated with the rice blast fungus Pyricularia oryzae. As expected, several plant protectants against the fungal invasion also induce a rise in PKC.

Journal

Citations (1)*help

See more

References(22)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top