Preparation and characterization of neem oil nanoemulsion formulations against Sitophilus oryzae and Tribolium castaneum adults

Choupanian Marziyeh, Omar Dzolkhifli, Basri Mahiran, Asib Norhayu

doi:10.1584/jpestics.d17-032

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ココクゾウムシおよびコクヌストモドキ成虫に対するニームオイルのナノエマルジョン製剤化による効果
Preparation and characterization of neem oil nanoemulsion formulations against Sitophilus oryzae and Tribolium castaneum adults

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This study aimed to improve the efficacy of azadirachtin (Azadirachta indica. A. Juss) against two serious pest species of stored products, Sitophilus oryzae (L.) and Tribolium castaneum (Herbst), through nano-emulsion formulations. Pseudoternary phase diagrams were constructed consisting of an emulsion system of an active ingredient (neem oil), surfactant (polysorbate or alkylpolyglucoside), and water. Isotropic regions were formed in the pseudoternary phase diagrams, and four formulations were selected from the isotropic regions and characterized according to particle size, particle aging, zeta potential, stability and thermostability, surface tension, viscosity, and pH. The selected formulations showed particle sizes of 208–507 nm in diameter. The result of contact toxicity demonstrated excellent mortality of S. oryzae and T. castaneum adults, with a mortality range of 85–100% and 74–100%, respectively, at a 1% azadirachtin concentration after only 2 days of exposure. Compared to non-formulated neem oil, the nano-emulsion formulations significantly increased the mortality of the tested species.

Journal

Journal of Pesticide Science

Journal of Pesticide Science 42 (4), 158-165, 2017

Pesticide Science Society of Japan

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Preparation and characterization of neem oil nanoemulsion formulations against <i>Sitophilus oryzae</i> and <i>Tribolium castaneum</i> adults