Identification of New Self-incompatibility Alleles (S-RNases) and Molecular S-genotyping of Genetic Resources in Loquat [Eriobotrya japonica (Thunb.) Lindl.]

Ishimoto Keiichiro, Fukuda Shinji, Nakayama Hisayuki, Hiehata Naofumi

doi:10.2503/hrj.13.11

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ビワ［Eriobotrya japonica（Thunb.）Lindl.］の新規自家不和合性遺伝子（S-RNase遺伝子）の同定および遺伝資源のSハプロタイプ推定
ビワ[Eriobotrya japonica (Thunb.) Lindl.]の新規自家不和合性遺伝子(S-RNase遺伝子)の同定および遺伝資源のSハプロタイプ推定
ビワ[Eriobotrya japonica (Thunb.) Lindl.]ノシンキジカフワアイショウイデンシ(S-RNase イデンシ)ノドウテイオヨビイデンシゲンノ Sハプロタイプスイテイ
Identification of New Self-incompatibility Alleles (S-RNases) and Molecular S-genotyping of Genetic Resources in Loquat [Eriobotrya japonica (Thunb.) Lindl.]

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Abstract

Some cultivars of loquat [Eriobotrya japonica (Thunb.) Lindl.; Rosaceae, Pyrinae] show gametophytic self-incompatibility controlled by a single, multi-allelic S locus, as in Japanese pear and apple. The S-genotype of loquat is an important factor for crossing and breeding. In this study, we tested a method to predict the S-genotype of some loquats by sequencer analysis. Sequencing revealed ten different band sizes. The nucleotide and amino acid sequences of five of these bands were identical to S-RNase sequences of loquat previously reported. Those of the other five bands were different from previous reports, but showed high-level homology with S-RNase sequences of apple and pear, and which conserved key cysteine and histidine residues. We conclude that the different sequences are likely to be new S alleles. The predicted S-genotypes of about 145 loquat cultivars and lines could be classified into 25 types. We confirmed that the main cultivars in Japan—‘Mogi’, ‘Tanaka’, and ‘Nagasakiwase’—are self-compatible at the gene level. The results suggest that the 41 cultivars and lines were self-incompatible.

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Horticultural Research (Japan)

Horticultural Research (Japan) 13 (1), 11-17, 2014

THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE

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Identification of New Self-incompatibility Alleles (<i>S-RNases</i>) and Molecular <i>S</i>-genotyping of Genetic Resources in Loquat [<i>Eriobotrya japonica</i> (Thunb.) Lindl.]