Cross-genera Amplification of <i>Brassica rapa</i> L. Microsatellite Markers in ‘Sakurajima daikon’ (<i>Raphanus sativus</i> L.) and its Application in the F<sub>1</sub> Purity Test

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  • ‘桜島大根’における<i>Brassica rapa</i>由来マイクロサテライトマーカーの属間増幅とそのF<sub>1</sub> 純度検定への応用
  • '桜島大根'におけるBrassica rapa由来マイクロサテライトマーカーの属間増幅とそのF₁純度検定への応用
  • 'サクラジマ ダイコン'ニ オケル Brassica rapa ユライ マイクロサテライトマーカー ノ ゾク カン ゾウフク ト ソノ F ₁ ジュンド ケンテイ エ ノ オウヨウ
  • Cross-genera Amplification of Brassica rapa L. Microsatellite Markers in ‘Sakurajima daikon’ (Raphanus sativus L.) and its Application in the F1 Purity Test

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Abstract

We have been conducting F1 hybrid breeding in ‘Sakurajima daikon’ to improve the uniformity of the variety. An F1 purity test using PCR-RFLP has been proposed in F1 variety Brassica vegetables; however, laborious and time-consuming restriction enzyme treatment is necessary in the test. One type of DNA marker, the microsatellite marker, has been widely used in crop genetics because of its convenience and marked reproducibility. We conducted an F1 purity test using microsatellite markers and investigated polymorphisms of 300 microsatellite markers derived from Brassica rapa L. in parental lines and their trial F1 crosses of ‘Sakurajima daikon’. We identified 8 markers that were highly polymorphic. Two of the markers that were detectable with agarose gel electrophoresis could be used to examine the purity of 3 elite F1 crosses in the field performance test.

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