Effects of Altericidins on the Leakage of Cellular Constituents from <i>Alternaria kikuchiana</i> and <i>Ustilago maydis</i>

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  • KIRINUKI Takeyoshi
    Faculty of Agriculture, Kobe University
  • ICHIBA Tsuneo
    Faculty of Agriculture, Kobe University Present address: Plant and Animal Science Development, Shionogi & Co., Ltd.

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Other Title
  • ナシ黒斑病菌およびトウモロコシ黒穂病菌の細胞内容物漏出に及ぼすアルテリシジンの影響
  • ナシ コクハンビョウキン オヨビ トウモロコシ クロホビョウキン ノ サイボウ
  • Mode of Action of Altericidins (Part 3)

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Abstract

The leakage of cellular constituents from Alternaria kikuchiana and Ustilago maydis treated with altericidins was investigated. Altericidins induced the leakage of 14C-labeled substances from mycelia and conidia of A. kikuchiana or sporidia of U. maydis preincubated with 14C-protein hydrolysate or 14C-glucose. The large amount of labeled substances leaked out into ambient solution within the first 1hr of incubation. The leakage of protein from mycelia and anthrone-positive substance from conidia of A. kikuchiana was also induced remarkably by altericidins. The former leakage reached the maximum 4 to 6hr after incubation with 20ppm altericidins and the latter 4hr after incubation with 8ppm altericidins. In the case of sporidia of U. maydis, the leakage of ninhydrin-positive substance, anthrone-positive substance and protein from the cells was clearly induced by 4ppm altericidins. The leakage of cellular constituents corresponded to the observation by scanning electron microscope reported previously. These results suggest that altericidins act on cell wall of fungi, induce change of the membrane permeability, and consequently kill them.

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