Labor-saving Preparation Method of Pollen Suspension for Artificial Pollination of Kiwifruit

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  • キウイフルーツの溶液受粉における効率的花粉懸濁液調整法
  • キウイフルーツ ノ ヨウエキ ジュフン ニ オケル コウリツテキ カフン ケンダクエキ チョウセイホウ

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Abstract

A new labor-saving method for preparing pollen suspension was developed for spray pollination of kiwifruit. Fresh anthers were dried on paper for anthesis. The dried anthers were directly suspended in the liquid extender (0.1% agar, 5% sucrose) and filtered through a 0.12 mm mesh. Sufficient pollen density in the suspension was obtained using more than 8% anthers (w/v) on a fresh weight basis. The pollen density of the preparation was determined by measuring the suspension at 400 nm using a spectrophotometer. There were no significant differences in fruit set, fruit weight, number of seeds or fruit quality in ‘Hayward’ when efficacy was compared between the newly developed preparation and the suspension prepared from pollen grains (0.5% [w/v]) that we formerly developed. The new preparation will eliminate the step previously performed to collect pollen grains from dried anthers and is thus a more labor-saving method for the artificial pollination of kiwifruit.<br>

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