Validation of Marker-Assisted Selection of Loquat Canker (Group A)-Resistant Seedlings with SSR Markers Obtained from Interspecies Crosses

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  • Ishimoto Keiichiro
    Fruit Tree and Tee Research Division, Agriculture and Forestry Technical Development Center, Nagasaki Prefectural Government
  • Fukuda Shinji
    Fruit Tree and Tee Research Division, Agriculture and Forestry Technical Development Center, Nagasaki Prefectural Government
  • Yamamoto Toshiya
    NARO Institute of Fruit Tree Science
  • Terakami Shingo
    NARO Institute of Fruit Tree Science
  • Hiehata Naofumi
    Fruit Tree and Tee Research Division, Agriculture and Forestry Technical Development Center, Nagasaki Prefectural Government
  • Tanimoto Emiko
    Fruit Tree and Tee Research Division, Agriculture and Forestry Technical Development Center, Nagasaki Prefectural Government

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Other Title
  • 種間交雑によって得られたビワがんしゅ病（Aグループ菌）抵抗性遺伝子座に連鎖するSSRマーカーのビワ育種における有用性
  • タネ カン コウザツ ニ ヨッテ エラレタ ビワ ガンシュヤマイ(Aグループキン)テイコウセイ イデンシザ ニ レンサ スル SSR マーカー ノ ビワイクシュ ニ オケル ユウヨウセイ

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Loquat canker is one of the most serious diseases of commercial varieties of loquat (Eriobotrya japonica). The pathogens are classified into three groups (A, B, and C) based on the production of a brown pigmentation in culture medium and pathogenicity to mesophyll. For marker-assisted selection (MAS) of Group A-resistant seedlings, we developed new simple sequence repeat (SSR) markers and fine-mapped the loquat canker-resistant gene Pse-a of bronze loquat (E. deflexa). The high-density linkage map included the Pse-a gene and 9 SSR markers, spanning 9.0 cM, with an average density of 0.9 cM per marker. The genotypes of these SSR markers were compared with the resistant phenotype of loquats from Japan (43 cultivars), other countries (13 cultivars), and unknown (1 cultivar). We found that two resistance-specific markers, SSR0254 and SSR0858, corresponded well with the resistant phenotype. The recombination values in two seedling populations between the marker and Pse-a were 2.3 and 12.6% for SSR0254, and 4.6 and 10.3% for SSR0858, respectively. Seedlings predicted to be resistant on the basis of SSR0254 or SSR0858 were resistant in an inoculation test with a probability of more than about 90% in every cross combination. We conclude that these markers may be useful in loquat breeding programs for MAS of individuals resistant to Group A.

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