A comparison of osteogenesis-related gene expression of mesenchymal stem cells during the osteoblastic differentiation induced by Type-I collagen and/or fibronectin
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- Ozawa Ryotaro
- Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya University School of Medicine
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- Yamada Yoichi
- Department of Center for Genetic and Regenerative Medicine, Nagoya University Graduate School of Medicine, Nagoya University School of Medicine
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- Nagasaka Tetsuro
- Department of Laboratory Medicine, Nagoya University Graduate School of Medicine, Nagoya University School of Medicine
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- Ueda Minoru
- Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya University School of Medicine
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説明
Mesenchymal stem cells (MSCs) contain multipotent cells and differentiate into osteoblasts, chondrocytes, and adiphocytes. Recently, MSCs have been used clinically in a tissue-engineering concept, but cell activity varies. It was found that the characteristics of extracellular matrices, type Tcollagen and/or fibronectin matrices, induced the osteoblastic differentiation of MSCs. Six weeks after cells were cultured with type I collagen and fibronectin, they formed mineralized tissue six times higher than other matrices. In this study, using “real time RT-PCR,” we assessed the expression of osteogenesis-related genes (alkaline phosphatase, BMP-2, osteocalcin, and cbfa-1) during osteoblastic differentiation by measuring their mRNA, quantitatively. The expression of the alkaline phosphatase gene increased time-dependently during osteoblastic differentiation until day 12, but expression induced by type I collagen and type I collagen and fibronectin decreased gradually. The expression of osteocalcin and the BMP-2 gene increased time-dependently; osteocalcin gene expression induced by type I collagen and fibronectin was about 6.2 times higher than non-induced cells. Expression of the cbfa-1 gene was different from that of type I collagen, fibronectin, and non-induced cells from day 12 and increased time-dependently. These findings were supported by alkaline phosphatase staining and immunofluoresence with an osteopontin antibody.
収録刊行物
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- ＩＪＯＭＳ
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ＩＪＯＭＳ 1 (2), 139-146, 2003
日本大学松戸歯学部 口腔科学研究所
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詳細情報 詳細情報について
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- CRID
- 1390001205213000064
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- NII論文ID
- 110004313858
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- NII書誌ID
- AA11830559
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- ISSN
- 21854254
- 13479733
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可