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- YAMAMOTO Ryohei
- Department of Advanced Pathobiology, Graduate School of Life & Environmental Sciences, Osaka Prefecture University
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- YAMAMOTO Mizuki
- Department of Advanced Pathobiology, Graduate School of Life & Environmental Sciences, Osaka Prefecture University
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- KUSAKA Hiroyuki
- Department of Advanced Pathobiology, Graduate School of Life & Environmental Sciences, Osaka Prefecture University
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- MASATSUGU Hideaki
- Department of Advanced Pathobiology, Graduate School of Life & Environmental Sciences, Osaka Prefecture University
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- MATSUYAMA Satoshi
- Department of Advanced Pathobiology, Graduate School of Life & Environmental Sciences, Osaka Prefecture University
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- TAJIMA Tomoko
- Department of Infectious Diseases Control, Graduate School of Life & Environmental Sciences, Osaka Prefecture University
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- IDE Hiroshi
- Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
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- KUBO Kihei
- Department of Advanced Pathobiology, Graduate School of Life & Environmental Sciences, Osaka Prefecture University
この論文をさがす
抄録
Oxidized pyrimidines are mainly repaired by base excision repair, which is initiated by damage-specific DNA glycosylases. NEIL1, the mammalian homolog of Escherichia coli endonuclease VIII and a major DNA glycosylase, initiates repair of oxidized pyrimidines. Here, we investigated the expression of two putative variant mouse NEIL1 (mNEIL1) mRNAs—variant 1 ("Neil1 protein" mRNA; BC043297 in the NCBI database) and variant 2 ("unnamed protein" mRNA; AK040802 in the NCBI database)—in normal mouse organs. Reverse transcription-PCR showed that both mRNAs were expressed in total RNA samples from 9 organs. Immunoblot analysis of a nuclear extract from normal mouse liver revealed three bands corresponding to full-length mNEIL1 protein and the two predicted variant proteins. However, neither variant protein, which included an N-terminal enzymatic activity domain deduced from the mRNA variants, were enzymatically active under multiple reaction conditions when expressed as his-tagged recombinant proteins. Nevertheless, recombinant variant 1 protein influenced mNEIL1 activity, while recombinant variant 2 protein had no influence. These results suggest that mNEIL1 mRNA variants are expressed in a variety of organs in normal mice and that variant 1 protein may regulate mNEIL1 activity.
収録刊行物
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- Journal of Radiation Research
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Journal of Radiation Research 53 (2), 234-241, 2012
Journal of Radiation Research 編集委員会
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詳細情報 詳細情報について
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- CRID
- 1390001205215059200
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- NII論文ID
- 130001876820
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- NII書誌ID
- AA00705792
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- COI
- 1:STN:280:DC%2BC38rlsl2htQ%3D%3D
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- ISSN
- 13499157
- 04493060
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- NDL書誌ID
- 023572158
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- PubMed
- 22510596
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可